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KMID : 0903520070500040358
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2007 Volume.50 No. 4 p.358 ~ p.361
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Antioxidant Activity of Isolated Compounds from the Heartwoods of Rhus verniciflua
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Ahn Eun-Mi
Park Sang-Jae
Choi Won-Cheol
Choi Suk-Hoon
Baek Nam-In
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Abstract
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The heartwoods of Rhus verniciflua was extracted with H2O and the concentrated extract was par- titioned with CHCl3, EtOAc, n-BuOH and H2O, successively. From the EtOAc and n-BuOH frac- tions, four compounds were isolated through the repeated silica gel, ODS and Sephadex LH-20 column chromatographies. They were determined as sinapyl aldehyde (1), 2,4-dihydroxybenzalde- hyde (2), 2,4-dihydroxybenzoic acid (3) and 2,4-dihydroxyphenylglyoxylic acid (4) on the basis of spectroscopic data, respectively. Among the isolated compounds, sinapyl aldehyde (34.7 ± 0.6%) and 2,4-dihydroxyphenylglyoxylic acid (43.8 ± 0.9%) showed the strong antioxidative activity than arti- ficial antioxidant BHT (14.4 ± 0.3%) in DPPH radical scavenging activity.
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KEYWORD
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Rhus verniciflua, phenolic compound, DPPH, antioxidative activity
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